E previously showed that the KXD/E motif binds COPI coatomers | D1T
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E previously showed that the KXD/E motif binds COPI coatomers to retain AtEMP12 in the Golgi of Arabidopsis (Gao et al., 2012). To examine whether the KXD/E motif has similar COPI-binding ability in other organisms, we used synthetic peptides corresponding to the AtEMP12 CT as bait to perform pull-down assays using proteins from yeast cell extracts followed by SDS AGE separation and tandem mass sp
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